Laboratory Research Technician IV

We are seeking a highly motivated individual for a full-time Research Specialist position in Dr. Hun-Way Hwang’s laboratory in the Department of Pathology. Our current research focuses on the role of abnormal mRNA processing in human disease. We combine powerful in vivo high-throughput profiling methods and CRISPR technology with genetically modified mouse models of human disease to identify candidate mRNA isoforms that may contribute to disease pathogenesis in specific cell types in vivo. These candidate mRNA isoforms will then be further studied by in vitro and in vivo methods to reveal their biological functions and the molecular mechanisms regulating their processing, which will provide novel insights into the management and treatment of human disease. Our two recent publications describe how cancer cells switch mRNA isoforms to survive chemotherapy (https://doi.org/10.1016/j.celrep.2021.109815) and how hyperactive mTORC1 alters mRNA isoform expression in tuberous sclerosis complex (https://elifesciences.org/articles/85036). Additional information can be found at https://www.hwangrnalab.org.Applicants should have a Bachelor’s degree in Biology or related field. Significant undergraduate lab experience beyond coursework and familiarity with the basics of molecular biology and mammalian cell culture is desired.

Performs a variety of independent and complex laboratory work and experiments. Analyzes and interprets data, compiles results, and prepares reports. Maintains inventory, ordering, and logs. Writes manuscripts and grant proposals and prepares data visualizations.

- Perform mammalian cell culture and molecular biology experiments- Maintain day-to day operation of the lab including preparing common lab reagents, ordering, shipping, filing inventories, tracking ongoing projects, equipment purchase/maintenance/repair, maintain IACUC and lab safety protocols- Optimal coordination of lab resources and experiments necessary for maximum productivity- Assist in mouse colonies maintenance and mouse experiments including regular visits to the mouse facility, breeding and weaning, genotyping and harvesting mouse tissues.

Little physicial effort required. Incumbent will be walking back and forth between various laboratory areas. Occasionally might need to lift water or buffer containers.

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